Genetic techniques and circuit analysis

Genetic techniques and circuit analysis

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  • Author: William Wisden
  • Publisher: Frontiers E-books
  • ISBN: 2889190269
  • Category :
  • Languages : en
  • Pages : 150

How new genetic techniques are revolutionizing the study of neural circuits for both invertebrate and vertebrate systems. Understanding how specific types of neurons contribute to behaviour is an ambitious goal. For invertebrate model systems (e.g. worms, flies), neurons in the brain are often too small to be studied routinely by electrophysiological approaches. For vertebrates, large ensembles of cells have to be studied, and these cells are often distributed over considerable volumes e.g. GABAergic interneurons in neocortex. Cell type-selective manipulations may be a way forward for treating illness. Before such aims can be realized, or even appreciated as feasible, the brain circuitry in experimental animals has to be known by both establishing the connections between cell types and reversibly manipulating the activity of the cells subtype-selectively. Methods that have all appeared in just the last couple of years to tackle this include: retrograde tracing of circuitry using viruses, ligand-receptor combinations that make subtypes of neurons uniquely sensitive to a drug (e.g. zolpidem, allatostatin, serotonin ligands or ivermectin), and light-activated channels and pumps for stimulation and inhibition. This collection of methods promises much, forming the new subdisciplines of “pharmacogenetics” and “opticogenetics”. These methods are revolutionizing the study of brain circuitry for both invertebrates and vertebrate systems.


Engineering Genetic Circuits

Engineering Genetic Circuits

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  • Author: Chris J. Myers
  • Publisher: CRC Press
  • ISBN: 1420083252
  • Category : Medical
  • Languages : en
  • Pages : 302

This text presents the modeling, analysis, and design methods for systems biology. It discusses how to examine experimental data to learn about mathematical models, develop efficient abstraction and simulation methods to analyze these models, and use analytical methods to design new circuits. The author reviews basic molecular biology and biochemistry principles, covers several methods for modeling and analyzing genetic circuits, and uses phage lambda as an example throughout to help illustrate the methods. He also explores the emerging area of synthetic biology. iBioSim software, lecture slides, and a password-protected solutions manual are available on the author's website.


Molecular-Genetic and Statistical Techniques for Behavioral and Neural Research

Molecular-Genetic and Statistical Techniques for Behavioral and Neural Research

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  • Author: Robert T. Gerlai
  • Publisher: Academic Press
  • ISBN: 0128041161
  • Category : Science
  • Languages : en
  • Pages : 710

Molecular-Genetic and Statistical Techniques for Behavioral and Neural Research presents the most exciting molecular and recombinant DNA techniques used in the analysis of brain function and behavior, a critical piece of the puzzle for clinicians, scientists, course instructors and advanced undergraduate and graduate students. Chapters examine neuroinformatics, genetic and neurobehavioral databases and data mining, also providing an analysis of natural genetic variation and principles and applications of forward (mutagenesis) and reverse genetics (gene targeting). In addition, the book discusses gene expression and its role in brain function and behavior, along with ethical issues in the use of animals in genetics testing. Written and edited by leading international experts, this book provides a clear presentation of the frontiers of basic research as well as translationally relevant techniques that are used by neurobehavioral geneticists. - Focuses on new techniques, including electrocorticography, functional mapping, stereo EEG, motor evoked potentials, optical coherence tomography, magnetoencephalography, laser evoked potentials, transmagnetic stimulation, and motor evoked potentials - Presents the most exciting molecular and recombinant DNA techniques used in the analysis of brain function and behavior - Written and edited by leading international experts


Synthetic Gene Circuits

Synthetic Gene Circuits

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  • Author: Filippo Menolascina
  • Publisher: Humana
  • ISBN: 9781071610343
  • Category : Medical
  • Languages : en
  • Pages : 353

This volume provides clear and direct protocols to implement automated Design-Build-Test-Learn (DBTL) into synthetic biology research. Chapters detail techniques to model and simulate biological systems, redesign biological systems, setting up of an automated biolaboratory, step-by-step guide on how to perform computer aided design, RNA sequencing, microfluidics -using bacterial cell free extracts, live mammalian cells, computational and experimental procedures, metabolic burden, computational techniques to predict such burden from models, and how DNA parts can be engineered in mammalian cells to sense, and respond to, and intracellular signals in general. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Synthetic Gene Circuits: Methods and Protocols aims to ensure successful results in the further study of this vital field.


The Science and Applications of Synthetic and Systems Biology

The Science and Applications of Synthetic and Systems Biology

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  • Author: Institute of Medicine
  • Publisher: National Academies Press
  • ISBN: 0309219396
  • Category : Science
  • Languages : en
  • Pages : 570

Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.


Advances in Genetics

Advances in Genetics

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  • Author:
  • Publisher: Academic Press
  • ISBN: 0128040793
  • Category : Science
  • Languages : en
  • Pages : 198

Advances in Genetics provides the latest information on the rapidly evolving field of genetics, presenting new medical breakthroughs that are occurring as a result of advances in our knowledge of genetics. The book continually publishes important reviews of the broadest interest to geneticists and their colleagues in affiliated disciplines, critically analyzing future directions. - Critically analyzes future directions for the study of clinical genetics - Written and edited by recognized leaders in the field - Presents new medical breakthroughs that are occurring as a result of advances in our knowledge of genetics


Genetic Design Automation

Genetic Design Automation

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  • Author: Hasan Baig
  • Publisher: Springer Nature
  • ISBN: 3030523551
  • Category : Technology & Engineering
  • Languages : en
  • Pages : 171

This textbook introduces readers to the recent advances in the emerging field of genetic design automation (GDA). Starting with an introduction and the basic concepts of molecular biology, the authors provide an overview of various genetic design automation tools. The authors then present the DVASim tool (Dynamic Virtual Analyzer and Simulator) which is used for the analysis and verification of genetic logic circuits. This includes methods and algorithms for the timing and threshold value analyses of genetic logic circuits. Next, the book presents the GeneTech tool (A technology mapping tool for genetic circuits) and the methods developed for optimization, synthesis, and technology mapping of genetic circuits. Chapters are followed by exercises which give readers hands-on practice with the tools presented. The concepts and algorithms are thoroughly described, enabling readers to improve the tools or use them as a starting point to develop new tools. Both DVASim and GeneTech are available from the developer’s website, free of charge. This book is intended for a multidisciplinary audience of computer scientists, engineers and biologists. It provides enough background knowledge for computer scientists and engineers, who usually do not have any background in biology but are interested to get involved in this domain. This book not only presents an accessible basic introduction to molecular biology, it also includes software tools which allow users to perform laboratory experiments in a virtual in-silico environment. This helps newbies to get a quick start in understanding and developing genetic design automation tools. The third part of this book is particular useful for biologists who usually find it difficult to grasp programming and are reluctant to developing computer software. They are introduced to the graphical programming language, LabVIEW, from which they can start developing computer programs rapidly. Readers are further provided with small projects which will help them to start developing GDA tools.


Decoding Neural Circuit Structure and Function

Decoding Neural Circuit Structure and Function

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  • Author: Arzu Çelik
  • Publisher: Springer
  • ISBN: 3319573632
  • Category : Medical
  • Languages : en
  • Pages : 517

This book offers representative examples from fly and mouse models to illustrate the ongoing success of the synergistic, state-of-the-art strategy, focusing on the ways it enhances our understanding of sensory processing. The authors focus on sensory systems (vision, olfaction), which are particularly powerful models for probing the development, connectivity, and function of neural circuits, to answer this question: How do individual nerve cells functionally cooperate to guide behavioral responses? Two genetically tractable species, mice and flies, together significantly further our understanding of these processes. Current efforts focus on integrating knowledge gained from three interrelated fields of research: (1) understanding how the fates of different cell types are specified during development, (2) revealing the synaptic connections between identified cell types (“connectomics”) using high-resolution three-dimensional circuit anatomy, and (3) causal testing of how iden tified circuit elements contribute to visual perception and behavior.


Cellular Computing

Cellular Computing

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  • Author: Martyn Amos
  • Publisher: Oxford University Press
  • ISBN: 0195155394
  • Category : Computers
  • Languages : en
  • Pages : 239

The view of the genome as a network of interacting computational components is well-established, but researchers try to reverse the analogy, by using living organisms to construct logic circuits. This book deals with the implementation of this technology, describing working experimental demonstrations using cells as components of logic circuits.


Uncommon Dissent

Uncommon Dissent

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  • Author: William Dembski
  • Publisher: Open Road Media
  • ISBN: 1497648955
  • Category : Science
  • Languages : en
  • Pages : 360

Recent years have seen the rise to prominence of ever more sophisticated philosophical and scientific critiques of the ideas marketed under the name of Darwinism. In Uncommon Dissent, mathematician and philosopher William A. Dembski brings together essays by leading intellectuals who find one or more aspects of Darwinism unpersuasive. As Dembski explains, Darwinism has gathered around itself an aura of invincibility that is inhospitable to rational discussion—to say the least: “Darwinism, its proponents assure us, has been overwhelmingly vindicated. Any resistance to it is futile and indicates bad faith or worse.” Indeed, those who question the Darwinian synthesis are supposed, in the famous formulation of Richard Dawkins, to be ignorant, stupid, insane, or wicked. The hostility of dogmatic Darwinians like Dawkins has not, however, prevented the advent of a growing cadre of scholarly critics of metaphysical Darwinism. The measured, thought-provoking essays in Uncommon Dissent make it increasingly obvious that these critics are not the brainwashed fundamentalist buffoons that Darwinism’s defenders suggest they are, but rather serious, skeptical, open-minded inquirers whose challenges pose serious questions about the viability of Darwinist ideology. The intellectual power of their contributions to Uncommon Dissent is bracing.