Challenges and Opportunities of Next-Generation Sequencing for Biomedical Research

2012-10-22
Challenges and Opportunities of Next-Generation Sequencing for Biomedical Research

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  • Author: Mark I. Rees
  • Publisher: Academic Press
  • ISBN: 012394287X
  • Category : Science
  • Languages : en
  • Pages : 210

Published continuously since 1944, the Advances in Protein Chemistry and Structural Biology serial has been a continuous, essential resource for protein chemists. Covering reviews of methodology and research in all aspects of protein chemistry, including purification/expression, proteomics, modeling and structural determination and design, each volume brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics. This volume features articles on Challenges and Opportunities of Next-Generation Sequencing for Biomedical Research. Covers reviews of methodology and research in all aspects of protein chemistry Brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics

Next Generation Sequencing

2016-01-14
Next Generation Sequencing

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  • Author: Jerzy Kulski
  • Publisher: BoD – Books on Demand
  • ISBN: 9535122401
  • Category : Medical
  • Languages : en
  • Pages : 466

Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.

Next Generation Sequencing Technologies in Medical Genetics

2013-10-16
Next Generation Sequencing Technologies in Medical Genetics

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  • Author: C. Alexander Valencia
  • Publisher: Springer Science & Business Media
  • ISBN: 1461490324
  • Category : Medical
  • Languages : en
  • Pages : 94

This book introduces readers to Next Generation Sequencing applications in medical genetics. The authors discuss the direct application of next-generation sequencing to medicine, specifically, laboratory medicine or molecular diagnostics. The first part of the book contains chapters on sanger sequencing, NGS technologies, targeted-amplification and capture, and exome sequencing. The second part of the book focuses on genetic disorders diagnoses by NGS, prenatal diagnosis, muscular dystrophies, mitochondrial disorders diagnosis, and challenges in molecular diagnosis. Recent developments and potential future trends in NGS sequencing applications are highlighted, as well.​

Next Generation Sequencing Technologies and Challenges in Sequence Assembly

2014-04-19
Next Generation Sequencing Technologies and Challenges in Sequence Assembly

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  • Author: Sara El-Metwally
  • Publisher: Springer Science & Business
  • ISBN: 1493907158
  • Category : Science
  • Languages : en
  • Pages : 118

The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field. This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.

Microbial Genomics in Sustainable Agroecosystems

2019-11-23
Microbial Genomics in Sustainable Agroecosystems

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  • Author: Vijay Tripathi
  • Publisher: Springer Nature
  • ISBN: 981329860X
  • Category : Science
  • Languages : en
  • Pages : 377

Today, microbiology is a rapidly growing discipline in the life sciences, and the technologies are evolving on a virtually daily basis. Next-generation sequencing technologies have revolutionized microbial analysis, and can help us understand the biology and genomic diversity of various bacterial species with significant impacts on agro-ecosystems. In addition, advances in molecular biology and microbiology techniques hold the potential to improve the productivity and sustainability of agriculture and forestry. This new volume addresses the role of microbial genomics in understanding the living systems that exist in the soil and their interactions with plants, an aspect that is also important for crop improvement. The topics covered focus on a deeper and clearer understanding of how microbes cause diseases, the genome-based development of novel antibacterial agents and vaccines, and the role of microbial genomics in crop improvement and agroforestry. Given its scope, the book offers a valuable resource for researchers and students of agriculture and infectious biology.

Next-Generation Sequencing Data Analysis

2016-04-06
Next-Generation Sequencing Data Analysis

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  • Author: Xinkun Wang
  • Publisher: CRC Press
  • ISBN: 1482217899
  • Category : Mathematics
  • Languages : en
  • Pages : 258

A Practical Guide to the Highly Dynamic Area of Massively Parallel SequencingThe development of genome and transcriptome sequencing technologies has led to a paradigm shift in life science research and disease diagnosis and prevention. Scientists are now able to see how human diseases and phenotypic changes are connected to DNA mutation, polymorphi

Progress and Challenges in Precision Medicine

2016-12-22
Progress and Challenges in Precision Medicine

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  • Author: Mukesh Verma
  • Publisher: Academic Press
  • ISBN: 0128095024
  • Category : Medical
  • Languages : en
  • Pages : 344

Progress and Challenges in Precision Medicine presents an insightful overview to the myriad factors of personalized and precision medicine. The availability of the human genome, large amounts of data on individual genetic variations, environmental interactions, influence of lifestyle, and cutting-edge tools and technologies for big-data analysis have led to the age of personalized and precision medicine. Bringing together a global range of experts on precision medicine, this book collects previously scattered information into one concise volume which covers the most important developments so far in precision medicine and also suggests the most likely avenues for future development. The book includes clinical information, informatics, public policy implications, and information on case studies. It is a useful reference and background work for students, researchers, and clinicians working in the biomedical and medical fields, as well as policymakers in the health sciences. Provides an overview of the growing field of precision medicine Contains chapters from geographically diverse experts in their field Explores important aspects of precision medicine, including applications, ethics, and development

Next Generation Sequencing

2013-05-31
Next Generation Sequencing

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  • Author: Lee-Jun C. Wong
  • Publisher: Springer Science & Business Media
  • ISBN: 1461470013
  • Category : Medical
  • Languages : en
  • Pages : 302

In recent years, owing to the fast development of a variety of sequencing technologies in the post human genome project era, sequencing analysis of a group of target genes, entire protein coding regions of the human genome, and the whole human genome has become a reality. Next Generation Sequencing (NGS) or Massively Parallel Sequencing (MPS) technologies offers a way to screen for mutations in many different genes in a cost and time efficient manner by deep coverage of the target sequences. This novel technology has now been applied to clinical diagnosis of Mendelian disorders of well characterized or undefined diseases, discovery of new disease genes, noninvasive prenatal diagnosis using maternal blood, and population based carrier testing of severe autosomal recessive disorders. This book covers topics of these applications, including potential limitations and expanded application in the future. ​

Applications of RNA-Seq and Omics Strategies

2017-09-13
Applications of RNA-Seq and Omics Strategies

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  • Author: Fabio Marchi
  • Publisher: BoD – Books on Demand
  • ISBN: 9535135031
  • Category : Medical
  • Languages : en
  • Pages : 330

The large potential of RNA sequencing and other "omics" techniques has contributed to the production of a huge amount of data pursuing to answer many different questions that surround the science's great unknowns. This book presents an overview about powerful and cost-efficient methods for a comprehensive analysis of RNA-Seq data, introducing and revising advanced concepts in data analysis using the most current algorithms. A holistic view about the entire context where transcriptome is inserted is also discussed here encompassing biological areas with remarkable technological advances in the study of systems biology, from microorganisms to precision medicine.

Next Generation Sequencing in Cancer Research

2013-08-04
Next Generation Sequencing in Cancer Research

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  • Author: Wei Wu
  • Publisher: Springer Science & Business Media
  • ISBN: 1461476453
  • Category : Medical
  • Languages : en
  • Pages : 383

​​​​This volume provides an interdisciplinary perspective of applying Next Generation Sequencing (NGS) technology to cancer research. It aims to systematically introduce the concept of NGS, a variety of NGS platforms and their practical implications in cancer biology.This unique and comprehensive text will integrate the unprecedented NGS technology into various cancer research projects as opposed to most books which offer a detailed description of the technology. This volume will present true experimental results with concrete data processing pipelines, discuss the bottleneck of each platform for real project in cancer research. In additional, single cancer cell sequencing as the proof of concept will be introduced in this book, along with cutting-edge information provided will help the intended audience to develop a comprehensive understanding of the NGS technology and practical whole genome sequencing data analysis and rapidly translate into their own research, specifically in the field of cancer biology.